CdX(X=Se,Te,Te/ZnS)量子点脂质体的制备及毒性比较

Preparation of CdX(X=Se,Te,Te/Zns) Quantum Dots Liposome and Comparison of Toxicity in Vitro and in Vivo

在水相中以巯基乙酸(mercaptoacetic acid,MA)为稳定剂合成了CdSe、CdTe、CdTe/ZnS量子点及谷胱甘肽(glutathione,GSH)为稳定剂合成了CdTe量子点,然后通过卵磷脂和胆固醇修饰制得相应的量子点脂质体。溶血实验证实GSH修饰量子点的溶血率低于MA修饰的量子点45%;脂质体修饰后,量子点的溶血率<5%,达到生物医用材料要求。不同表面修饰的量子点对小鼠毒性存在明显差异,荧光显微镜观察组织切片证实量子点在小鼠体内主要分布在肺、肾、胸腺等组织中,而脂质体量子点在脑组织中富集明显。

Mercaptoacetic acid（MA） capped CdSe, CdTe, CdTe/ZnS quantum dots（QDs） and glutathione（GSH） QDs were prepared in water phase; then quantum dots liposome （QD-LP） were synthesized by modifying QDs surface with lecithin and cholesterol. In vitro hemolysis tested, the hemolysis rate of GSH-capped QDs was lower 45% than MAcapped QDs, and QD-LP was less than 5%, which meet the requirements of biomedical materials. The toxicity in vivo was tested with tissue section by fluorescence microscope. It showed that different surface-modified QDs had different toxicity in mice. QDs were distributed mainly in the lung, kidney and thymus tissues, but QD-LP mainly existed in the brain tissues.