三七总皂苷对泡沫细胞内ABCA1和LXRα mRNA表达的影响

Effects of Panax notoginseng saponins on mRNA expressions of ABCA1 and LXRα in foam cells

目的观察三七总皂苷(Panaxnotoginsengsaponins,PNS)对泡沫细胞ABCA1和LXRαmRNA表达及脂质沉积的影响。方法提取SD大鼠腹腔巨噬细胞纯化后,与高、中、低剂量PNS(80、40、20μg/ml)及氧化低密度脂蛋白共孵育48h。油红O染色观察细胞内脂质堆积情况,酶法测定细胞内总胆固醇(TC)、游离胆固醇(FC)、胆固醇酯(CE)的含量,并计算CE/TC值。采用RT-PCR法检测泡沫细胞ABCA1和LXRαmRNA表达情况。结果模型组细胞中TC、FC、CE含量及CE/TC值均显著高于对照组(P<0.05);与模型组相比,PNS高、中剂量组细胞内TC、FC、CE含量及CE/TC值均显著降低(P<0.05)。PNS高、中剂量组中ABCA1及LXRαmRNA的表达均显著高于模型组(P<0.01)。结论PNS可以上调ABCA1和LXRαmRNA表达并减少巨噬细胞源性泡沫细胞中脂质沉积。

Objective To observe the effects of Panax notoginseng saponins （PNS） on mRNA expressions of ABCA1 and LXRα and lipid deposition in foam cells. Methods Peritoneal macrophages obtained from SD rats after purification were incubated with ox-LDL for 48 h to establish foam cell model. PNS （20, 40, 80 μg/ml, respectively） were given for intervention. The cellular lipid accumulation was examined by oil red staining. The cellular contents of total cholesterol （TC）, free cholesterol （FC） and cholesterol ester （CE） and the ratio of CE/TC were detected by enzymic methods. The mRNA levels of ABCA1 and LXRα were determined by RT-PCR. Results The contents of TC, FC, CE and CE/TC ratio in model group were significantly increased compared with control group （ P 〈 0.05 ）. Compared with model group, 40 and 80 μg/ml PNS reduced the contents of TC, FC, CE and CE/TC ratio remarkably （p 〈 0. 05） and increased ABCA1 and LXRα mRNA expressions significantly （p 〈0.01 ）. Conclusion PNS reduces lipid deposition and upregulates ABCA1 and LXRα mRNA expressions in foam cells.