CXCR4／SDF-1对鼻咽癌细胞增殖与迁移能力的影响

Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines

目的观察鼻咽癌不同恶性程度的细胞中趋化因子受体CXCR4的表达,检测CXCR4/SDF-1反应轴对鼻咽癌细胞的增殖作用,SDF-1对CXCR4阳性肿瘤细胞的趋化作用,探讨CXCR4/SDF-1生物学轴对人鼻咽癌细胞增殖与迁移能力的影响。方法以鼻咽癌成瘤高转移细胞株5-8F及成瘤不转移细胞株6-10B为研究对象,采用Westernblot免疫印迹法检测鼻咽癌细胞株CXCR4蛋白的表达情况,SDF-1及CXCR4阻断剂AMD3100作用于两种细胞后,MTT法检测细胞的增殖能力,体外迁徙实验检测CXCR4/SDF-1反应轴对鼻咽癌细胞的趋化作用。结果5-8F细胞株CXCR4蛋白的表达明显高于6-10B细胞株;SDF-1能明显增强5-8F细胞增殖与迁移能力,CXCR4阻断剂AMD3100作用后,5-8F细胞增殖与迁移能力明显降低;SDF-1对6-10B细胞的迁移及增殖能力无明显影响。结论CXCR4/SDF-1生物学轴与鼻咽癌细胞株的增殖与迁移有一定的关系,AMD3100可明显抑制鼻咽癌细胞的增殖与迁移能力。

Objective To detect the expression of chemokine receptors 4 （CXCR4） in human nasopharyngeal carcinoma cell lines （NPC cells） and explore the role of CXCR4/stromal cell-derived factor-1 （ SDF-1 ） axis in the proliferation and migration of tumor cell. Methods The expression of CXCR4 protein in NPC cell lines 5-8F （with high tumogenicity and metastasis ） and 6-10B （having tumogenicity but no metastasis） was measured by Western blot analysis. After the addition of SDF-1 and CXCR4 blocker, AMD3100, the changes of cell growth were observed by MTT. The changes of cell migration were measured by Transwell. Results Western blotting showed that 5-8F cells expressed higher CXCR4 levels than 6-10B cells. The growth rate and the number of migration cells in 5-8F cells was increased after SDF-1 treatment, and significantly decreased after the addition of AMD3100. However, 6-10B cells had no response to SDF-1. Conclusion CXCR4/SDF-1 axis may play an important role in NPC cells proliferation and migration, and CXCR4 blocker can efficiently suppress this effect.