应用微量中和试验进行猪伪狂犬病血清学调查

The Serum Epidemical Survey on Swine Pseudorabies by Micro Neutralization Test

应用细胞培养微量中和试验（固定病毒稀释血清法）对来自２４个猪场共４２６份血清进行了猪伪狂犬病血清学调查。共检出阳性血清１７１份，血清阳性率达４０．１％，出现血清阳性的猪场有２０个，占所检测猪场的８３．３％，说明伪狂犬病在我省及我国流行相当严重。对其中５０份血清的中和指数（固定血清稀释病毒法）进行了检测，结果中和效价大于１∶２血清的中和指数都在１０２．５以上。

The samples of 426 swine sera derived from 24 pig farms were detected for the neutralizing antibodies against swine pseudorabies Virus by micro neutralization test.The positive rate of sera is up to 40 1%(171/426),and the positive rate of the farm amounts to 83 3%(20/24).The results indicated that Pseudorabies was epidemic severely in Hubei Province.At the same time,the neutralization index of 50 sera which neutralization titer is more than 2 was detected to be over 316.