期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

高丹草SSR引物设计及分子遗传框架图谱构建 被引量：12

SSR Primer Designing and Construction of a Genetic Map of Sorghum bicolor×S. sudanense
下载PDF
导出
摘要 利用高粱EST序列和BAC克隆,以Primer 5.0程序设计了10对高丹草SSR引物,结果发现有3对引物在皖草2号高丹草的亲本间表现多态性,其中2对来自BAC的引物可用于遗传连锁图谱构建。除以上10对SSR引物外,再选取已公布的245对高粱SSR引物,以180个皖草2号(TX623A×S722)的F2代单株作为构图群体,构建了一个包括4个连锁群、33个SSR标记的高丹草遗传图谱,图谱覆盖基因组长度458.5cM,平均图距13.8cM。 Ten SSR primer pairs derived from EST and BAC in sorghum were developed using the software Primer 5.0 and 3 of them were polymorphic between Sorghum bicolor TX623A and S. sudanense S722, 2 of them were used for construction of genetic map. A genetic map of Sorghum bicolor × S. sudanense was constructed based on 10 SSR primer pairs developed and 245 publicly available SSR primer pairs for sorghum with a population of 180 F2 individuals derived from a cross between TX623A and S722. The map consisted of 4 linkage groups and 33 SSR makers. The map covered 458.5cM with average genetic distance of 13.8cM.
作者 卢杰 吕媛媛 李杰勤 詹秋文 王敏
机构地区 安徽科技学院 安徽农业大学
出处 《中国草地学报》 CSCD 北大核心 2009年第2期28-33,共6页 Chinese Journal of Grassland
基金 科技部农业科技成果转化资金项目(No:2008GB2C300125) 国家科技支撑计划项目(No:2008BADB3B3B10) 安徽省“十一五”科技攻关计划重点项目(No:06013104B) 安徽省高校学科拔尖人才基金(No:教秘人〔2005〕79号) 安徽省自然科学基金资助项目(No:01041102)
关键词 高丹草 SSR 引物设计 遗传图谱 构建 Sorghum bicolor × S. sudanense SSR Primer designing Genetic map Construction
分类号 S544 [农业科学—作物学] Q943.2 [生物学—植物学]
  • 相关文献

参考文献26

  • 1Fengshan l.iang, Qiyun Deng. Yueguang Wang, Yuedong Xiong, Demin Jin.Jiming Li,Bin Wang. Molecular marker assisted selection for yiel&cnhancing genes in the progeny of "9311 × O. rufipogon"using SSR[J]. Ellph ytica, 2001,139 (2) : 159-165.
  • 2Assar A H Abu,Uptmoor R,Abdelmula A A,SaIih M,Ordon F,Friedt W. Genetic variation in sorghum germplasm from Su dan. ICRISAT, and USA assessed by Simple Sequence Repeats (SSRs)[J]. Crop Sci. ,2005,45:1636 1644.
  • 3Panaud O,Chen X,McCouch S R.Development of microsatellite markers and characterization of simple sequence length polymorphism(SSLP)in rice (Oryza satia L.)[J].Malecular Geneties and Genomics, 1996,252(5) :597 -607.
  • 4Yu J K, Rota M I., Kantety R V, Sorrells M E. EST derived SSR markers for comparative mapping in wheat and rice[J]. Molecular Genetics and Genomics, 2004,271(6) : 742-751.
  • 5Thiel T, Michalek W, Varshney R,Graner A. Exploiting EST data- bases for the development and characterization of gene-derived SSR markers in barley (Hordeum vulgare L. ) [J]. Theor Appl Genet, 2003,106(3) :411- 422.
  • 6dbEST release 012006. Summary by organism [EB/OL]. January 20, 2006. http://www, ncbi. nlm. nih. gov/dbEST/dbEST_ summary. html. [2008-06-06].
  • 7Bhattramakki D,Dong J, Hart G E,Chhabra A K. An integrat- ed SSR and RFLP linkage map of Sorghum bicolor (L.) Moench[J]. Genome, 2000,43 : 988-1002.
  • 8Kong L,Dong J, Hart G E. Characteristics, linkage map positions, and allelic differentiation of Sorghum bicolor (L.) Moench DNA simple sequence repeats (SSRs)[J]. Theor Appl Genet, 2000,101(3) :438-448.
  • 9Menz M A,Klein R R, Mullet J E,Obert J A,Unruh N C, Klein P E. A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers [J]. Plato Molecular Biology, 2002,48 : 483-499.
  • 10Wu Y Q, Huang Y. An SSR genetic map of Sorghum bicolor (L.) Moench and its comparison to a published genetic map [J]. Genome, 2007,50( 1 ) :84-89.

二级参考文献96

  • 1钱章强,詹秋文.杂交草2号的选育及栽培要点[J].科学养鱼,1997,19(1):39-39. 被引量：11
  • 2Ajisaka H,Kuginuki Y,Shiratori M,Ishiguro K,Enomoto S and Hirai M.Mapping of loci affecting the cultural efficiency of microspore culture of Brassica rapa L.syn.Campestris L.using DNA polymorphism (J).Breeding Science,1999,49:187～192
  • 3Bentolia S,Hardy T,Guitton C,Freyssinet G,Busso C,Stenhouse J and Ottaviano E.Similarity of maize and sorghum genomes as revealed by maize RFLP probes (J).Theoretical Applied Genetics,1992,84:10～16
  • 4Brummer E C,Bouton J H and Kochert G.Development of anRFLP map in diploid alfalfa (J).Theoretical Applied Genetics,1993,86:329～332
  • 5Buckler E S,Phelps-Durr IV T L,Buckler C S K,Dawe R K,Doebley J F and Holtsford T P.Meiotic drive of chromosomal knobs reshaped the Maize genome (J).Genetics,1999,42:326～335
  • 6Byrne M,Murrell J C,Allen B and Moran G F.An integrated genetic linkage map for eucalypts using RFLP,RAPD and isozyme markers (J).Theoretical Applied Genetics,1995,91:869～875
  • 7Charlesworth B.Driving genes and chromosomes (J).Nature,1988,332:394～395
  • 8Cheng R,Saito A,Takano Y and Ukai Y.Estimation of the position and effect of a lethal factor locus on a molecular marker linkage map (J).Theoretical Applied Genetics,1996,93:494～502
  • 9Cloutier S,Cappadocia M and Landry B S.Study of microspore-culture responsiveness in oilseed rape (Brassica hapus L.) by comparative mapping of a F2 population and two microspore-derived populations (J).Theoretical Applied Genetics,1995,91:841～847
  • 10Cloutier,S,Cappadocia,M and Landry,B S Analysis of RFLP mapping inaccuracy in Brassica napus L (J).Genetics,1997,95:83～91

共引文献637

同被引文献151

引证文献12

二级引证文献64

中国草地学报
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部