一种快速有效制备单克隆抗体的方法

A Rapid and Efficient Preparation for Monoclonal Antibodies

以人绒毛膜促性腺激素（ｈＣＧ）为抗原，对ＢＡＬＢ／ｃ小鼠一次脾内直接注射免疫，免疫后６ｄ，取其血清进行抗体检测，结果１２８００倍稀释度仍呈阳性。取此免疫小鼠脾细胞与同系小鼠骨髓瘤细胞ＮＳ－１进行细胞融合。融合后，将经检测抗体阳性的细胞孔置于显微镜下，挑选单个克隆进行克隆化，得到２株单克隆抗体细胞株２Ａ８、４Ｅ６。将其分别接种于ＢＡＬＢ／ｃ小鼠腹腔中，收获的腹水效价分别达１０－６和１０－５。

BALB/c mice were immunized once by intrasplenic injection of hCG. On 5th day after immunization, the sera were collected and assayed, the sera titer of more than 1∶12 800 was determined to be positive. Spleen cells of the positive mice were fused with myeloma cells NS 1, and single clone was selected and cloned from the growing cell well detected as antibody positive after fusion. Two monoclone lines, 2A8 and 4E6, were obtained and then separately injected into the abdominal cavity of BALB/c mice, with the titers of the abdominis hydrops being 10 -6 and 10 -5 respectively.