黄芪多糖对雏鸡外周血T淋巴细胞转化功能的影响

Effect of Astragalus Polysaccharide(APS) on T Lymphocyte Transformation Function of Peripheral Blood in Chickens

将１０８只１日龄伊莎系蛋用公雏均分为３组：一组为对照，其余２组在３日龄时，于背侧颈部皮下分别注射０．２、０．４ｍＬ黄芪多糖注射液（０．０１ｇ／ｍＬ）１次，再分别于７、２１、３５、４９日龄时采用ＭＴＴ比色法及微量全血培养３Ｈ－ＴｄＲ掺入法检测外周血Ｔ淋巴细胞转化率的动态变化。结果表明，黄芪多糖对２１、３５日龄雏鸡Ｔ淋巴细胞转化功能有增强作用，且与剂量有相关性，而对７、４９日龄雏鸡的作用不明显。ＭＴＴ比色法与３Ｈ－ＴｄＲ掺入法的检测结果无显著差异（Ｐ＜０．０５）。

Experiments were carried out to determine the effect of astragalus polysaccharide(APS) on the T lymphocyte transformation function in chickens. The tansformation rates of peripheral T lymphocyte were measured respectively by MTT colorimetric assay and 3H TdR incorporation microassay with whole blood at 7 , 21 , 35 and 49 day old. The results showed that APS enhanced the function of cell mediated immunity in chickens of 21, 35 day old but did not of 7, 49 day old and there was a positive relationship between the enhanced function and the dose of APS; There was no significant difference between the two methods (P<0.05).