摘要
目的:调查普通成年人群隐匿性HBV感染率。方法:收集普通成年人血清1440份,ELISA检测HBV血清学标志物,分别设计HBV DNA不同区域的引物,用巢式PCR检测HBV DNA,对阳性标本进行DNA序列分析,并确定HBV基因型。结果:1440份血清中,HBsAg阳性70例(4.86%);HBsAg阴性的1370份血清中,3例(0.21%)至少2组不同特异性引物巢式PCR显示HBV DNA阳性。其DNA序列与基因库中已知HBV序列同源,证实了PCR产物的特异性,而各HBV DNA序列又不完全相同,排除了因污染造成的假阳性。3例隐匿性HBV感染中2例为基因型B型,另1例为C型。结论:普通成年人群存在隐匿性HBV感染。输血和器官移植时,仅以血清学标志物作为筛查HBV的手段,可能会引起HBV的传播。
Objective:To investigate the positive rate of occult hepatitis B virus(HBV) infection in general adults.Methods:HBV serological markers in the sera of 1440 adults were detected by ELISA.The sera negative for HBsAg were subjected to detecting HBV DNA with nested PCR using various sets of primers derived from S and C regions respectively,followed by sequencing the PCR products.Results:Of 1440 adults,70(4.86%) were positive for HBsAg.Among other 1370 negative for HBsAg,3(0.21%) were HBV DNA positive,which was detected at least by two PCR assays using different primers.Sequencing data demonstrated that they were highly homologous to the HBV DNA sequence in GenBank while none of them was identical to the known sequences detected in this laboratory,confirming the specificity of the amplified HBV DNA.Of the three occult HBV infections,two were HBV genotype B and another was genotype C.Conclusion:Occult HBV infection does exist in adult population.The potential infectivity of such occult HBV infection in blood transfusion or organ transplantation should be considered.
出处
《中国卫生检验杂志》
CAS
2009年第3期495-497,502,共4页
Chinese Journal of Health Laboratory Technology
基金
南京市科技发展项目(200801088)资助课题