高效液相色谱法测定板栗罐头中乙二胺四乙酸二钠的含量

Determination of ethylene diamine tetraacetic acid disodium salt in canned chinese chestnut by high performance liquid chromatography

目的：建立板栗罐头中乙二胺四乙酸二钠含量的高效液相色谱测定方法。方法：样品经水提取,加入络合剂络合后,以C8色谱柱（250 mm×4.6 mm i.d 5μm）进行分离,以含12.5%甲醇、0.13%的四丁基氢氧化铵和0.052%甲酸的水溶液（pH3.5）作为流动相,流速为1.00 ml/min,检测波长为254 nm。结果：本方法回收率为94.6%～101.6%,RSD为1.96%～3.65%。结论：本方法简便快捷,重现性较好,干扰少,用于板栗罐头中乙二胺四乙酸二钠测定,取得满意结果。

Objective：To establish a method for determination of disodium ethylene diamin tetraacetate in canned chinese chestnut using high performance liquid chromatography.Methods：By competition coordination with metal ion Fe（Ⅲ）.After precipitation by water and competition coordination with FeCl3,The sample was separated on a C8 column（250 mm×4.6 mm i.d 5 μm）.The mobile phase was 12.5%（v/v） methanol solution containing 0.13%（v/v） tetrabutyl ammonium hydroxide and 0.052%（v/v） formic acid（pH=3.5）.Disodium ethylene diamin tetraacetate was detected at 254 nm,with flow rate of 1.00 ml/min.Results：The relative standard deviation（RSD） of peak areas of disodium ethylene diamin tetraacetate was 1.96%～3.65%,The recoveries of disodium ethylene diamin tetraacetate tanged from 94.6% to 101.6%.Conclusion：The simple and rapid method with good reproducibility can be used for the determination of disodium ethylene diamin tetraacetate in canned chinese chestnut.