正相色谱法测定食品中三聚氰胺的研究

Study on determination of melamine in food by high-performance liquid chromatography

目的：建立食品中三聚氰胺的高效液相色谱测定方法。方法：采用紫外检测器,利用费罗门Luna NH2氨基色谱柱（250 mm×4.6 mm i.d.,5μm）,以90%（v/v）乙腈为淋洗液,流速1.0 ml/min,采用波长240 nm进行检测,外标法定量。结果：三聚氰胺在相应的试验浓度范围内均具有良好的线性关系,其相关系数均大于0.999,最低检出限为1.25 mg/kg,平均回收率在82.5%～99.4%之间,RSD均小于5.0%。结论：该法具有简单、准确、灵敏等优点,适合食品中三聚氰胺含量的检测。

Objective：To establish an analytical method for the determination of melamine in food by high-performance liquid chromatography.Methods：The melamine was separated on Luna NH2 column（250 mm×4.6 mm i.d.,5 μm）by using 90% acetonitrile as an eluent at a flow rate of 1.0 ml/min.Detection was performed on an ultraviolet detector.The wavelength was 240 nm.Quantification was calculated by an external standard method.Results：The linearity in the tested concentration range was good and the correlation coefficient was more than 0.9990.The limit of quantification was 0.1 mg/kg for the target compounds.The extraction recoveries were 82.5%～99.4% and the RSD less than 5.0%.Conclusion：This method is simple,sensitive and accurate for the determination of the melamine in food and it can be satisfactorily applied to the routine analysis.