高效液相色谱-紫外检测法测定尿中α-萘酚、β-萘酚和1-羟基芘

Simultaneous determination of 1-naphthnal,2-naphthnal and 1-hydroxypyrene in urine by high liquid chromatography-ultraviolet photometric dection

目的：建立了一种同时检测尿中α-萘酚、β-萘酚和1-羟基芘（1-OHP）的反相高效液相色谱的方法。方法：采用Diamonsil^TMC18（5μm,150×4.6 nm）色谱柱,以V（甲醇）：V（乙酸铵缓冲液）=70：30为流动相,在波长280 nm处检测,流速为0.7 ml/min。结果：当α-萘酚、β-萘酚和1-羟基芘的质量浓度分别为0.3600～18.72 mg/L,0.3600～18.72 mg/L,0.2700～11.88 mg/L时,峰高和质量浓度呈很好的线性关系,相对标准差分别为0.9%、2.2%、3.2%（n=5）,平均回收率分别为100.3%、103.1%、99.4%。结论：方法准确快速,可用于人尿中α-萘酚、β-萘酚和1-羟基芘的同时测定。

Objective：To develop a reversed-phase high performance liquid chromatography method for assaying 1-naphthol,2-naphthol and 1-hydroxypyrene（1-OHP）.Methods：1-naphthol,2-naphthol and 1-OHP were separated on Diamonsil^TMC18（5 μm,150×4.6 nm）and detected at 280 nm by using methanol-ammonium acetate buffer solution（70：30,v：v） as mobile phase when the column temperature was 35.0℃ and the flow rate was 0.7 ml/min.Results：The calibration curves of а-naphthol,β-naphthol and 1-OHP showed good linearities in the ranges of 0.3600～18.72 mg/L,0.3600～18.72 mg/L,0.2700～11.88 mg/L,respectively.The relative standard deviations（RSD） were 0.9%,2.2%,3.2%（n=5）and the average recoveries were 100.3%,103.1%,99.4% respectively.Conclusion：The method is accurate and suitable for the identification and quantification of these compositions in urine.