高效液相色谱法同时测定食品中的糖精钠、苯甲酸、山梨酸、富马酸二甲酯和脱氢乙酸钠

High-performance liquid chromatography determination in foods of saccharin sodium,benzoic acid,sorbic acid,dimethyl fumarate and dehydrogenation sodium acetate at same time

目的：研究一种快速、准确、简单的高效液相色谱同时测定烘烤食品中糖精钠、苯甲酸、山梨酸、富马酸二甲酯、脱氢乙酸钠的方法。方法：样品用甲醇溶解,经超声提取后,调pH值为6左右,选用Eclipse XDB-C18分离柱,用28＋72（V/V）的甲醇与乙酸铵-无水硫酸钠-三氟乙酸混合液作流动相。以保留时间定性,峰面积定量。结果：11 min内可较好的完成5种组分的分离。回收率在91.3%～105.3%之间,检出限分别为0.28、0.32、0.33、0.43、0.25（mg/L）。结论：该方法快速、简单、准确,可用于烘烤食品中糖精钠、苯甲酸、山梨酸、富马酸二甲酯、脱氢乙酸钠的同时测定。

Objective：A fast,accurate,simple and high-performance liquid chromatography was studied to the simultaneous determination of saccharin sodium,benzoic acid,sorbic acid,dimethyl fumarate and dehydrogenation sodium acetate solution baked foods.Methods：Samples with methanol dissolved,the ultrasonic extraction,for pH value of about 6,were separated on Eclipse XDB-C18 column,and eluted with a mixed of 28＋72（V/V） methanol and ammonium acetate-anhydrous sodium-trifluoroacetic acid mixture as mobile phase.To retain the qualitative of time,peak area or quantitative.Results：It could be better to complete the five components of the separation in 11 minutes.The recovery is 91.3%～105.3%,the detection limit were 0.28,0.32,0.33,0.43,0.25（mg/L）.Conclusion：This method is fast,simple,accurate and can be used to determine saccharin sodium,benzoic acid,sorbic acid,dimethyl fumarate and dehydrogenation sodium acetate solution in baked foods at the same time.