不同脱乙酰度壳寡糖促胰岛细胞增殖、胰岛素分泌及调节餐后血糖作用的研究

Antidiabetic Effects of Varying Degree of Deacetylation Chitooligosaccharides on Pancreatic Islet Cells in Streptozotocin-induced Diabetic Rats

试验旨在探讨N-乙酰化反应制备的不同脱乙酰度壳寡糖对胰岛β细胞系NIT-1的促增殖及胰岛素分泌作用,并进一步探讨在体内壳寡糖降低链脲佐菌素诱导的糖尿病大鼠餐后2h血糖的作用。通过N-乙酰化反应制备得到水溶性的不同脱乙酰度的壳寡糖,在细胞水平上,通过形态学观察、MTT比色法、放射免疫等方法研究不同浓度的壳寡糖对胰岛β细胞系NIT-1细胞的增殖及促胰岛素分泌作用;通过一般状态观察、餐后2h血糖、尿糖、糖耐量测定研究了不同脱乙酰度壳寡糖对链脲佐菌素诱导的糖尿病大鼠降低餐后2h血糖和改善葡萄糖耐量的作用。结果表明,对β细胞体外增殖具有明显的促进作用,并可以显著促进原代培养胰岛细胞的胰岛素分泌。因此,壳寡糖在体内能有效改善糖尿病大鼠的体重减轻、多饮、多食等症状,降低餐后2h血糖值和尿糖,改善葡萄糖耐量。

To detect the varying degree of deaeetylation effect of chitooligosaccharides prepared by enzymatic hydrolysis on the proliferation of pancreatic islet cells,the release of insulin and 2 h plasma glucose in diabetic mouse induced by streptozotocin. Chitooligosaccharides were prepared by enzymatic hydrolysis. In vitro, the effect of varying degree of deacetylation chitooligosaccharides on the proliferation of pancreatic islet cells and the release of insulin were detected with optical microscope,MTT colorimetric assay, radioimmunoassay methods respectively ; the general clinical symptom, 2 h plasma glucose, urine glucose, oral glucose tolerance test were examined after 60 days feeding study to determine the effect of varying degree of deacetylation chitooligosaccharides on diabetic mouse induced by streptozotocin. The results indicate that the varying degree of deacetylation chitooligosaccharides can effectively accelerate the proliferation of the pancreastic β cells, chitooligosaccharides has prominent effect on the release of insulin. All varying degree of deacetylation chitooligosaccharides have effective effects on the general clinical symptom of DM rats,decrease the 2 h plasma glucose and urine glucose, normalize the disorders of glucose tolerance.