期刊文献+

临宰生猪口蹄疫免疫抗体水平的检测与分析 被引量:1

The Detection and Analysis of Pigs' FMD Antibody Level before Slaughtered
下载PDF
导出
摘要 为了调查外省市调入东莞的临宰生猪口蹄疫抗体水平,2004年、2006年11月~12月、2007年2月~3月、2007年3月~4月共4个阶段,对调入东莞市的临宰生猪进行随机抽血采样,共2800份。第1阶段用ELISA方法检测,第2~4阶段用O型口蹄疫正向间接血凝试验检测抗体。结果表明,2004年抽样合格率仅为13.7%,2006年11月~2007年4月,临宰生猪免疫抗体高于1∶32的占29.77%,高于1∶128的只占9.66%,调查结果表明,调入东莞市的临宰生猪口蹄疫抗体水平普遍偏低,需加强生猪上市前口蹄疫的免疫。 The objective of this pepar is to investigate and study FMD antibody level of slaughtered pigs transported from foreign provinces. 2800 serum samples were got in Dongguan during the four stages of 2004, November 2006 to December 2006,February 2007 to March 2007 and March 2007 to April 2007, the samples of 2004 were detected by ELISA, the others were detected by FMDV type O positive hemagglutination. The qualified rate is only 13.7 % in 2004, antibody level above 1 : 32 is 29.77%, while above 1 : 128 only accounts for 9. 66% in November 2006 to April 2007. The results show that FMD antibody level is low.
出处 《中国畜牧兽医》 CAS 北大核心 2009年第3期160-162,共3页 China Animal Husbandry & Veterinary Medicine
关键词 临宰生猪 口蹄疫 免疫抗体 检测 分析 pig before slaughtered foot-and-mouth disease antibody detection analysis
  • 相关文献

参考文献4

二级参考文献58

  • 1杨永钦,黄德生,李乐.斑点ELISA检测口蹄疫病毒的研究[J].中国兽医杂志,1994,20(9):6-7. 被引量:12
  • 2杨永钦.生物素─亲和素强化斑点试验检测口蹄疫病毒抗体的研究[J].中国畜禽传染病,1994,16(5):12-15. 被引量:4
  • 3朱彩珠,卢永干,邱孝高,赵启祖,谢庆阁.反转录-聚合酶链反应检测牛食道-咽部分泌物中的口蹄疫病毒[J].中国兽医科技,1996,26(8):3-5. 被引量:3
  • 4[1]Bergmann I E,Malirat E V,Neitzert E,Beck N,et al.Improvement of a serodiagnostic strategy for foot-and-mouth disease virus surveillance in cattle under systematic vaccination:a combined system of an indirect ELISA-3ABC with an enzyme-linked immunoelectrotransfer blot assay [J].Arch Virol,2000,145:473~489.
  • 5[2]Diego D M,Brocchi E,Mackay D,et al.The non-structural polyprotein 3ABC of foot-and-mouth virus as a diagnostic antigen in ELISA to differentiate infected from vaccinated cattle [J].Arch Virol,1997,142:2021~2033.
  • 6[3]Mackay D K J,Forsyth M A,Davies P R,et al.Differentiating infection from vaccination in foot-and-mouth disease using a panel of recombinant non-structural proteins in ELISA [J].Vaccine,1997,16:446~459.
  • 7[4]Neitzert E,Beck E,Auge P,et al.Expression of the aphthovirus RNA polymerase gene in Escherichia coli and its use together with other bioengineered nonstructural antigens in detection of late persistent infections [J].Virology,1991,184:799~804.
  • 8[5]Rodriguez A,Dopazo J,Saiz J C,et al.Immunogenicity of non-structural proteins of foot-and-mouth disease virus:differences between infected and vaccinated swine [J].Arch Virol,1994,136:123~131.
  • 9[6]Silberstein E,Kaplan G,Taboga O,et al.Foot-and-mouth disease virus-infected but not vaccinated cattle develop antibodies against recombinant 3AB1 nonstructural protein [J].Arch Virol,1997,142:795~805.
  • 10[7]Sorensen K J,Madsen K G,Madsen E A,et al.Differentiation of infection from vaccination in foot-and-mouth by the detection of antibodies to the non-structural proteins 3D,3AB and 3ABC in ELISA using antigens expressed in baculovirus [J].Arch Virol,1998,143:1~16.

共引文献56

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部