摘要
目的：研究杂色曲霉素对人肺组织的致癌作用。方法：以组织培养方法研究了杂色曲霉素体外对人胚肺细胞致恶性转化作用。结果：发现杂色曲霉素处理４周后，体外培养的人胚肺细胞增殖旺盛，并出现细胞异型性变化，并可见复层交叉生长的转化灶。杂色曲霉素处理２４周，处理细胞可在软琼脂培养基上形成集落，杂色曲霉素１μｇ／ｍｌ、３μｇ／ｍｌ组平均集落数分别为１５．３、１６．１个／皿。
Objective:To study carcinogenic effect of sterigmatocystin(ST) on human lung tissue in vitro.Methods:By means of cellular culture,malignant transformation effect of ST was studied in vitro on lung cells of human fetus.Results:Active proliferation and cellular atypia were found after 4 weeks of ST treatment,crossgrowing multilayer malignant transformation foci appeared.Then 24 weeks after ST treatment,cellular colonies formed in soft agar,mean colony numbers for ST 1μg/ml and 3μg/ml group were 15.3 and 16.1 per dish respectively.Conclusion:ST could induce malignant transformation in human fetal lung tissue in vitro.
出处
《河北医科大学学报》
CAS
1998年第3期129-131,共3页
Journal of Hebei Medical University
基金
国家自然科学基金
卫生部优秀青年科技人才基金
关键词
肺肿瘤
病因学
杂色曲霉素
胎儿
sterigmatocystin
lung neoplasms/etiol
lung/growth
fetus
