摘要
目的:评价mdr1基因与Pgp糖蛋白在急性血病(AL)细胞中表达的相关性及其临床意义。方法:分别应用RTPCR及流式细胞术(FCM)检测32例AL患者白血病细胞中的mdr1mRNA及Pgp糖蛋白表达率及表达水平,同时检测14例正常人作为对照。结果:临床非耐药组mdr1阳性率为22.2%,Pgp阳性率为33.3%,而临床耐药组两者分别为78.6%、85.7%,均与临床治疗结果有极显著的一致性(P<0.005和P<0.01)。mdr1+/Pgp+的13例患者中,11例为临床耐药(84.6%),其中10例表现为mdr1+/Pgp+结果为中高度表达,临床非耐药的2例患者,mdr1+/Pgp+都是低度表达。相关性检验显示两方法有极显著的一致性。结论:mdr1与Pgp均可预测MDR,同时检测mdr1/Pgp可提高MDR检出符合率。
Objective:To study the relationship between multidrug resistance gene(mdr1) and Pglycoprotein(Pgp) in acute leukemic cells and evaluate their clinical significance.Methods:The expression levels of mdr1 mRNA and Pgp were studied by reverse transcription ploymerase chain reaction(RTPCR) and flow cytometry method(FCM) respectively in 32 cases of acute leukemia and 14 normal controls.Results:The mdr1 and Pgp positive rates were 22.2%,33.3% in clinical nonresistant group and 78.6%,85.7% in drugresistant group.The mdr1 and Pgp eapressions were in accordance with the results of clinical chemotherapy.Among 13 cases of acute leukemia showing positive mdr1 and Pgp expression,11 cases displayed clinical drug resistance and 10 of these cases expressed mdr1 and Pgp at medium and high levels.Only two cases in the clinical nonresistant group expressed mdr1 and Pgp at a lower level.There was significant correlation between the two methods.Conclusion:Both FCM analysis of Pgp and RTPCR detection of mdr1 could predict the multidrug resistance(MDR) and the results of the two methods showed a good correlation.The diagnostic accordance rate of MDR can be raised while mdr1 and Pgp are detected at the same time.
出处
《河北医科大学学报》
CAS
1998年第3期132-134,共3页
Journal of Hebei Medical University
关键词
白血病
MDR-1基因
P-gp糖蛋白
药物疗法
MDR
leukemia/drug ther
leukemia/metab
drug resistance
polymerase chain reaction
flow cytometry