采用聚合酶链反应检测肺结核病患者中的结核杆菌

POLYMERASE CHAIN REACTION IN DETECTION OF MYCOBACTERIUMTUBERCULOSIS IN PULMONARY TUBERCULOSIS PATIENTS

目的：建立一种快速、敏感及特异的早期诊断肺结核病方法。方法：采用聚合酶链反应（ＰＣＲ）技术，检测了６４例肺结核患者的外周血与痰标本结核杆菌ＤＮＡ。结果：外周血结核杆菌ＤＮＡ的阳性率为６７．２％（４３／６４）；痰标本的阳性率为２９．６％（１９／６４）（Ｐ＜０．０５）。结论：用ＰＣＲ技术诊断肺结核病。

Objective:It is to set up a quick,sensitive and specific method for diagnosis of early pulmonary tuberculosis.Methods:Polymerase chain reaction(PCR) was adopted in detection of DNA of mycobacterium tuberculosis from peripheral blood and sputum in 64 tuberculosis patients.Results:The positive rate of the DNA was 67.2% (43/64 cases) in peripheral blood and 29.6% (19/64 cases) in sputum(P<0.05).Conclusion:PCR can raise specificity and sensitivity of diagnosis in pulmonary tuberculosis.