人蛋白质二硫键异构酶活性片段基因的克隆及序列测定

Cloning and sequencing of the gene coding for human protein disulfide isomerase active fragment

目的:获取人蛋白质二硫键异构酶活性片段(hPDIf)基因,并进行序列测定,为今后研究其机理及应用创造条件.方法:从人胎肝组织中用反转录PCR方法扩增hPDTf活性片段基因,重组入pUC19质粒载体,用双脱氧法双向测定目的基因序列.结果:从人胎肝组织中成功地扩增到hPDIf基因,测出的序列与已知基因序列一致.结论:构建了hPDIf基因的重组克隆,为以后的深入研究奠定了基础.

To get human protein isomcrase active fragment (hPDIf) gene for exploring the action mechanism am application of hPDIf. Methods: The encoding gene of hPDIf was amplified by reverse PCR from human embryo-liver andcloned into plasmid pUC19. The nucleotide sequence of the targeted UNA was determined by the dideoxy chain termina tion method with forward and reverse primers. Results: The nucleotide sequence was consisted with the known gene. Con clusion: Recombinant hPDIf gene clone was established fo: further research and application.