用逆转录套式PCR方法对西安地区HGV感染情况的调查

Investigation of infection of HGV RNA by RT-nest-ed-polymerase chain reaction in Xi'an

目的:为了了解庚型肝炎病毒(HGV)在我国的感染状况,得到一较好的HGV检测方法.方法:采用两对GBV-C(HGV)NS_3区引物,用逆转录-套式-聚合酶链反应(RT-nested-PCR)和ELISA方法对67例非甲乙丙丁戊型急性肝炎患者血清进行了检测.结果:发现17例呈NGV RNA阳性,阳性率为25.4%,PCR与ELISA法进行抗-HGVIgG的检测的阳性符合率为18%,同时对HGV的检测方法进行讨论.结论:西安市急性肝炎患者中存在着一定程度的HGV感染.PCR特别是逆转录套式PCR方法在目前来说无疑是进行HGV检测特异性和灵敏度均较好的选择,可广泛用于临床和流行病学研究.

It is essential to provide a better method for detection of hepatitis G virus(HGV) to gain the information about HGV infection in our country. Methods; Nested-PCR and ELISA were employed by using two pairs of primers based on the conserved region NS3 of GBV-C(HGV) in 67 serum samples from non-A,non-B, non-C, non-D, non-E a-cute hepatitis patients. Results: HGV RNA was detected in 17 serum samples (25. 4%) and the coincident rate between PCR and ELISA was 18%, at the same time the detective methods about HGV were discussed. Conclusion: There are some HGVRNA positive in acute hepatitis in Xi'an. At pre-sent, PCR, especially RT-Nested-PCR is a highly sensitive and specific method for the detection of HGV in no doubt, it may be widely used to clinic and the study of epidemiology.