维甲酸诱导HL-60细胞Fas蛋白表达

Retinoic acid inducing Fas expression in HL-60 cells

目的：探讨全反式维甲酸（ＡＴＲＡ）和９顺式维甲酸（９ｃｉｓＲＡ）对ＨＬ６０细胞Ｆａｓ表达水平及维甲酸对抗Ｆａｓ抗体诱导的细胞凋亡敏感性的影响。方法：用流式细胞仪检测ＨＬ６０细胞膜Ｆａｓ蛋白表达水平；分别采用形态学观察、ＤＮＡ电泳和流式细胞仪检测抗Ｆａｓ抗体诱导的细胞凋亡。结果：ＨＬ６０细胞Ｆａｓ弱表达。经１０－６ｍｏｌ／ＬＡＴＲＡ或９ｃｉｓＲＡ分别预处理４天后，ＨＬ６０细胞Ｆａｓ表达水平及对抗Ｆａｓ抗体诱导凋亡的敏感性均显著提高。９ｃｉｓＲＡ提高ＨＬ６０细胞对抗Ｆａｓ抗体诱导凋亡的敏感性的能力强于ＡＴＲＡ。结论：维甲酸可上调ＨＬ６０细胞表达Ｆａｓ，这可能与维甲酸诱导ＨＬ６０细胞分化相关。进一步深入探讨其分子机制，将为肿瘤治疗。

Objective: To investigate the effect of alltrans retinoic acid (ATRA) and 9cis retinoic acid (9cis RA) on the expression of Fas and the sensitivity to antiFas induced apotosis in HL-60 cells. Methods: Fas expression was detected by flow cytometry. Anti-Fas induced apoptosis (AFIA) was determined by morphological features, DNA fragment electrophoresis, and flow cytometric cell cycle analysis. Results: Fas antigen was weakly expressed on HL-60 cells. After incubation of HL-60 cells with 10^-6mol/L ATRA or 9cis RA for 4 days, both the Fas expression and AFIA were significantly enhanced. 9cis RA was more potent than ATRA in increasing the sensitivity to AFIA. Conclusion: RA might upregulate Fas expression. Further elucidation of its molecular mechanisms might provide a rationale for new therapeutic strategies of malignant diseases.