维甲类化合物Ro13-7410对HL-60细胞凋亡及分化诱导作用

Induction of apoptosis and differentiation in human leukemia cell line HL-60 by synthetic retinoid Ro137410

目的：观察第三代维甲类化合物（Ｅ）４［２５，６，７，８ｔｅｔｒａｈｙｄｒｏ５，５，８，８ｔｅｔｒａｍｅｔｈｙｌ２ｎａｐｈｔｈａｌｅｎｙｌ１ｐｒｏｐｅｎｙｌ］ｂｅｎｚｏｉｃａｃｉｄ（Ｒｏ１３７４１０）对人白血病细胞系ＨＬ６０的影响。方法：应用剂量反应曲线，台盼蓝排斥法，光镜、电镜，凋亡细胞检测试剂盒以及硝基四氮唑蓝还原能力等测定观察Ｒｏ１３７４１０对ＨＬ６０细胞的作用。结果：Ｒｏ１３７４１０能明显抑制ＨＬ６０细胞增殖，并同时诱导ＨＬ６０细胞的凋亡和分化。结论：Ｒｏ１３７４１０作用于ＨＬ６０细胞后可使ＨＬ６０细胞发生凋亡和分化。

Objective: To investigate the effect of the synthetic retinoid Ro137410 on HL60 cells. Methods: Trypan blue exclusion was used to determine viability. Apoptosis was assessed by cell morphology and PCDassaykit. Differentiation was assessed by morphology and NBT reduction. Results: Incubation of the HL60 cells with 10-6～10-8mol/L Ro147410 resulted in the suppression of cell growth, the induction of cell apoptosis and differentiation. Conclusion: Ro137410 suppressed HL60 cell growth mainly via induction of apoptosis.