肥胖和肥胖抵抗大鼠脂肪组织PPARγ和aP2基因表达的研究

Study on PPARγ and aP2 mRNA expression of adipose tissues in obesity and obesity resistant rats

目的观察高脂饮食诱导的肥胖及肥胖抵抗大鼠脂肪组织中PPARγ和aP2 mRNA表达水平的变化。方法31只健康wistar雄性大鼠,随机选取7只作为对照组喂饲基础饲料,24只作为高脂组喂饲高脂饲料。第8周末按体重增量,从高脂组选出5只大鼠作为肥胖组,5只作为肥胖抵抗组,检测各组大鼠血清甘油三酯和总胆固醇,附睾周围脂肪组织中PPARγ和aP2 mRNA表达。结果肥胖组大鼠血清甘油三酯水平显著高于对照组(P<0.05);总胆固醇水平均显著高于对照组和肥胖抵抗组(P<0.05,P<0.01);肥胖组PPARγ和aP2 mRNA表达量高于对照组和肥胖抵抗组(P<0.05)。结论高脂饮食诱导产生的肥胖及肥胖抵抗大鼠在脂肪组织PPARγ和aP2 mRNA表达上存在差异,肥胖大鼠脂肪合成能力增强。

Objective To observe the difference of serum triglyceride, total cholesterol levels and expression of PPARγ and aP2 mRNA of adipose tissues between obesity and obesity resistant rats induced by high-fat diet. Methods Thirty-one healthy male Wistar rats were randomly divided into two groups. Seven of them were fed with standard diet as control group and 24 rats were fed with high-fat diet as high-fat group. At the end of the 8th week, according to the body weight increment, five rats were selected as obesity group, and five rats were selected as obesity resistant group from highfat feeding rats. Serum trlglyceride, total cholesterol, and the expressions of PPARγ and aP2 mRNA of epididymal adipose tissues were detected. Results Serum triglyceride of obesity group were higher than those of control （ P 〈 0.05）. Total cholesterol levels of obesity group were higher than those of control and obesity resistant group （ P 〈 0.05, P 〈 0.01 ）. The expressions of PPARγ and aP2 mRNA of obesity group were also much higher than those of control and obesity resistant group. Conclusion The expressions of PPARγ and aP2 mRNA of obesity and obesity resistant rats induced by high-fat diet existed difference.