葡萄砧木‘5BB’PGIP基因的克隆及生物信息学分析

Cloning and Analysis of PGIP Gene from Grape Rootstock ‘5BB’(Vitis berlandieri×V.riparia)

以葡萄砧木品种‘5BB’（Vitis berlandieri×V.ripria）为试材，通过设计特异引物、PCR扩增，从‘5BB’基因组中得到1条全长1002bp的多聚半乳糖醛酸酶抑制蛋白（PGIP）基因序歹11，该序列包含1个完整的开放阅读框，与欧洲葡萄、其它果树的相应序列同源性分别为99％和68％，其推导的蛋白质序列中包含一段亮氨酸重复序列，第1～27个氨基酸残基是信号肽，三级结构与菜豆PGIP相似。

Specific primers, which were synthesized according to the conservative regions of PGIP （polygalacturonase inhibiting protein） gene, were used to amplify genomic DNA from grape rootstock ＇5BB＇ （Vitis berlandier/ × V. riparia） by polymerase -chain reaction （PCR）. A sequence of PGIP gene with whole length of 1002 bp was obtained, which contained a complete open reading frame. Its DNA sequence was homologous to those from Vitis vinifera and other fruits by 99% and 68% respectively. Its protein sequence contained a typical leucine - repeated sequence. The predicted signal peptide was formed by 27 residues from 1 to 27, and the predicted PGIP three dimensional model was similar with that of Phaseolus vulgaris by using bioinformatics method and tool.