摘要
研究了虎杖嫩茎愈伤组织诱导和分化、不定芽的分化和生根、试管苗移栽和扦插及田间栽培所需要的条件,并建立起虎杖嫩茎的高效无性系及快速繁殖技术。结果表明:MS+6-BA0.4 mg/L+NAA1.2 mg/L是诱导形成具有分化能力愈伤组织的理想培养基;MS+AgNO30.8 mg/L+6-BA0.3 mg/L+NAA0.1 mg/L是愈伤组织和不定芽分化的理想培养基;1/3MS+IAA0.4 mg/L+NAA0.1 mg/L是不定芽生根培养和试管苗生根继代培养的理想培养基;炉灰渣是试管苗移栽和扦插的理想基质。移植的试管苗具有长势粗壮旺盛、春天发芽早、秋天枯萎晚、根系发达的性状,其他各种性状保持不变。
The conditions needed in callus induction and differentiation of tender stem, differentiation and rooting of adventitious bud, transplantation of test- tube seedlings, and field cultivation of Polygonum cuspidatum have been studied, and its regenerative clone and rapid propagation techniques were established. The results showed that the best medium for callus induction was MS ＋ 6 - BA 0.4 mg/L ＋ NAA 1.2 mg/L. The best medium for differentiation was MS ＋ AgNO3 0.8 mg/L ＋ 6 - BA 0.3 mg/L ＋ NAA 0. 1 rag/ L. 1/3MS ＋ IAA 0. 4 mg/L ＋ NAA 0. 1 mg/L was suitable for rooting and regeneration. Cinder was the ideal substrate for the transplantation of test-tube seedlings. Field planting results showed that the test-tube seedlings grew well with flourishing root system.
出处
《江西农业学报》
CAS
2009年第3期73-76,共4页
Acta Agriculturae Jiangxi
关键词
虎杖
愈伤组织
组织培养
无性系
快速繁殖
Polygonum cuspidatum
Callus
Tissue culture
Clone
Rapid propagation
