水稻抗细菌性条斑病菌离体叶片检验法影响因素研究

Studies on Influential Factors of Excised Leaf Tissue Inoculation of Bacterial Leaf Streak in Oryza sativa

对水稻细菌性条斑病抗性离体叶鉴定方法的影响因素进行了研究。结果表明：离体叶检验法需在琼脂培养基中加入75～100μL/mL的苯丙咪唑，以保持鉴定叶片非感染部位叶色为绿色；鉴定期间培养温度以25～30℃为宜；接种用茵液浓度不低于3×10^5CFU／mL；当菌液浓度较高时，潜育期的变化对温度不敏感，为5～6d；当菌液浓度与温度同时降低时，则潜育期延长。

The excised leaf tissue inoculation （ELTI） method was used to identify the resistance of rice to bacterial leaf streak in this paper. The results showed that ELTI was a more efficient and dependable method for disease-resistance identification than the traditional method. It was necessary to keep leaf green by adding 75～100μL/mL of benzimidazole into agar medium in the whole identification period. For efficient inoculation and identification of excised leaf tissue, the temperature was required to be 25～30℃, and the lowest concentration of bacterial solution should be 3×10^5 CFU/mL.