氯喹对烟雾吸入伤大鼠肺微血管通透性的影响

Influence of chlorqurine on pulmonary microvascular permeability in rats after smoke inhalation injury

目的探讨氯喹对烟雾吸入伤大鼠肺微血管通透性的影响。方法健康Ｗｉｓｔａｒ大鼠随机分成正常对照组，吸入伤１，３，５，１２，２４ｈ组及氯喹治疗６ｈ和１２ｈ组。肺含水量用干湿重法测定，肺微血管通透性用１３１Ｉ－白蛋白渗出量表示，肺出血量用９９Ｔｃ标记的红细胞出量表示。结果烟雾吸入伤后肺水含量、１３１Ｉ－白蛋白渗出量明显增加，至伤后６ｈ达峰值（Ｐ＜０．０１），而肺内９９Ｔｃ红细胞出量以伤后１ｈ最明显，伤后２４ｈ仍明显高于正常对照组（Ｐ＜０．０１），氯喹治疗伤后６ｈ组，上述指标明显低于吸入伤６ｈ组（Ｐ＜０．０１）。结论氯喹可以明显降低烟雾吸入伤大鼠肺微血管通透性。

AIM To explore the effect of chlorqurine on pulmonary microvascular permeability in rats after smoke inhalation injury. METHODS Wistar rats were randomly divided into control group, injured groups following smoke inhalation 1, 3, 6, 12, 24 h and treated groups with chlorqurine (10 mg·kg-1, immediately injection intraperitoneally after smoke inhalation) following smoke inhalation 6, 12 h, the content of lung water was measured with drywet weight, the pulmonary microvascular permeability was represented by 131Ilabelled albunim exudation, pulmonary hemorrhage was quantitated with 99mTclabelled erythocytes. RESULTS There were significant increases in the content of lung water and the exudation of 131Ilabelled albumin and reached peak level of 6 hour after smoke inhalation injury (P<001), the countered numbers of 99mTclabelled erythocytes was the highest at 1 hour following smoke inhalation and was higher at 24 hours after smoke inhalation compared with control group; there were obvious decreases in the content of lung water, the exudation of 131Ilabelled albumin and the countered numbers of 99mTclabelled erythocytes in chlorqurine treatment group at 6 hours following smoke inhalation compared with injoured group at 6 hours after smoke inhalation (P<001). CONCLUSION Chlorqurine might decreased the pulmonary microvascular permeability of rats after smoke inhalation injury.