姜黄素对大鼠心肌缺血性损伤的保护作用

Protective effects of curcumin on myocardial ischemic injury in rats

目的观察姜黄素对大鼠心肌缺血的保护作用，探讨可能的作用机制。方法用大剂量异丙肾上腺素（Ｉｓｏ）造成大鼠心肌缺血性损伤模型，观察姜黄素３个剂量组和对照组大鼠的心电图、血清酶（ＣＰＫ，ＬＤＨ，ＧＯＴ）活性和血清中游离脂肪酸（ＦＦＡ）含量以及血清和心肌组织中超氧化物歧化酶（ＳＯＤ）的活性和脂质过氧化产物丙二醛（ＭＤＡ）的含量的变化。结果姜黄素可使Ｉｓｏ诱导的大鼠心电图缺血性改变（Ｊ点下移）减轻，抑制血清ＣＰＫ，ＬＤＨ，ＧＯＴ活性的升高，抑制ＦＦＡ含量的升高，并可降低缺血心肌组织中ＭＤＡ的含量，且这些作用呈现一定的剂量依赖关系。结论姜黄素可提高大鼠心肌耐缺氧能力，对大鼠心肌的缺血性损伤具有一定的保护作用。该保护作用的机制可能与其清除自由基功能有关。

AIM To observe the effect of curcumin, an antioxidant, on myocardial ischemic injury in rats. METHODS Rat myocardial ischemia was induced by overdosage isoprenaline (Iso) injected subcutaneously. Curcumin was given 20 min before Iso injection. The changes of serum CPK, LDH, GOT and FFA were observed, and ECG and myocardial SOD and MDA detected. RESULTS The myocardial ischemic injury (changes of ECG, increase of serum CPK, LDH, GOT and FFA) was significantly attenuated and the myocardial content of MDA decreased in curcumin groups. The effects of curcumin were in a dosedependent manner. CONCLUSION Curcumin might enhance the torlerance of rat myocardium to hypoxia, and might have protective effect on ischemic injury of rat myocardium. The mechanism of the protective effect might be related to its free radical scavenging activity.