大黄素对角朊细胞体外增殖分化影响的研究

Study on effects of emodin on keratinocyte proliferation and differentiation in vitro

目的观察大黄素对角朊细胞体外增殖分化的影响。方法以人转化型角朊细胞株ＣＯＬＯ－１６细胞为模型，采用活细胞计数、３Ｈ－ＴｄＲ参入法、细胞因子生物活性检测、电镜及ＡＢＣ法进行研究。结果大黄素能以一种剂量依赖方式抑制角朊细胞的体外增殖和ＤＮＡ合成；减少角朊细胞肿瘤坏死因子－α（ＴＮＦ－α）、白细胞介素－６（ＩＬ－６）的产生；对角朊细胞超微结构有直接损伤作用；并抑制角朊细胞角蛋白表达，破坏细胞骨架。结论大黄素通过不同途径抑制角朊细胞的体外增殖分化，是角朊细胞增殖分化的有效抑制剂。

AIM The effects of emodin on keratinocyte proliferation and differentiation in vitro were observed. METHODS Using the culture of human transformed keratinocyte cell line COLO16 cells as a model, viable cell count, 3HTdR uptake, cytokine bioactivity assay, electron microscope examination and ABC method were applied in this study. RESULTS The inhibitory effects of emodin on keratinocyte proliferation and DNA synthesis were observed, and it was found that the inhibitory effects were dosedependent. In the supernatant dirived from COLO16 cells treated with emodin, tumor necrosis factorα (TNFα) and interleukin6 (IL6) production were significantly reduced. The membrane, mitochondrion and cytoskeleton of keratinocytes were severely damaged and the keratin expression was suppressed. CONCLUSION This study suggestes that the proliferation and differentiation of keratinocytes in vitro were suppressed by emodin through various ways, and emodin may be an effective suppressing drug on keratinocyte proliferation and differentiation.