反义c-myc寡脱氧核苷酸对大鼠腹主动脉损伤后内膜增生的影响

Effect of calcitonin gone related peptide on coronany thrombosis and thrombolytic therapy

目的观察大鼠腹主动脉损伤后内膜增生的影响，为采用反义ｃｍｙｃ战略防治再狭窄的早期临床试验奠定基础。方法将反义ｃｍｙｃ反义寡脱氧核苷酸（ＯＤＮ）通过ｐｌｕｒｏｎｉｃｇｅｌ缓释系统和Ｌｉｐｏｆｅｃｔｉｎ转染导入系统，将其作用于大鼠腹主动脉损伤后的血管外膜，饲养三周后，处死动物，对所取的血管节段进行组织切片和弹力纤维染色，在显微镜下进行形态观察和采用图像分析系统进行图像分析，计算出内膜／中膜（Ｉ／Ｍ）面积之比和Ｉ／Ｍ厚度之比，并进行统计学分析。结果通过形态观察和Ｉ／Ｍ面积和Ｉ／Ｍ厚度之比，发现反义ｃｍｙｃＯＤＮ明显抑制了内膜增生，而且随着反义ｃｍｙｃＯＤＮ剂量的增加，其对内膜增生的抑制作用更加明显，而正义ｃｍｙｃＯＤＮ和错配ｃｍｙｃＯＤＮ与对照组相比，对内膜增生无影响。结论反义ｃｍｙｃＯＤＮ以剂量依赖性和序列特异性方式抑制了新生内膜的形成。

Objective To inverstigate the effect of Caleitonin gene related peptide (CGRP) on intracoronary thrombosis, thrombolysis, and reocclusion. Methods Thirty six dogs were divided into three groups:saline solution (NS) group, nitroglycerin(NTG) group and CGRP group. The canine model of intracoronary thrombsis was established. Urokinase(UK) was administered intravenously. The time and rate of intracoronary thrombosis, patency and reocclusion were observed. Results The time of thrombus formation was markedly prolonged in the CGRP group. (NS 41±9min, CGRP 72±11min P <0.01). The time of patency was shorted from 80±7min(NS), 70±11min(NTG) to 50±9min(CGRP) ( P <0.01). The time of reocclusion was increased from 20±10min(NS),39±7min(NTG) to 65±12min(CGRP)( P <0.01). Conclusions CGRP can delay the intracoronary thrombus formation, assist thrombolysis and decrease the reocclusis after thrombolysis.