摘要
【目的】建立1种含脂辐照食品中特异性辐解产物2-十二烷基环丁酮的萃取分离以及定量分析的新方法。【方法】采用硅胶层析柱萃取分离、气相色谱-质谱联用检测,对淋洗剂浓度、硅胶活化程度、收集时间及气相色谱-质谱条件等技术参数进行优化。【结果】该方法对2-十二烷基环丁酮标准品的最低检出限为0.005mg·L-1,0.1~2.0mg·L-1的添加回收率为75.9%~103.9%,相对标准偏差(RSD)均小于5.7%。【结论】与欧盟标准方法EN1785进行比较,硅胶柱分离法具有成本低、提取效率高、重现性好、检出限低、承载样品量大等优点。
[Objective] A novel method was developed for quantitative determination of 2-dodecylcyclobutanone which is one of the specific markers of irradiated fat containing foods. [ Method] The method includes extraction and separation by silica gel column and analysis by capillary column gas chromatography-mass spectrometry (GC-MS). And the correlative parameters of the concentration of eluting solvent, extent of deactivated silica, and collecting time for separation and chromatogram detection were optimized. [Result] The limit of detection was 0.005 mg·L^-1, the recoveries were 75.9%-103.9% according to adding concentration of 0.1-2.0 mg·L^-1, and the relative standard deviations were all below 5.7%. [Conclusion] It showed that the silica gel column separation method has lower cost, higher separative efficiency, better stability, lower detection limit, and larger analytic sample as compared to the EN 1785 method.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第3期989-995,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(10475025)
国家“十一五”科技支撑计划(2006BAK02A19-5,2006BAK02A18-3)
关键词
2-十二烷基环丁酮
辐照
硅胶柱
气相色谱-质谱联用
2-dodecylcyclobutanone
irradiation
silica column
gas chromatography-mass spectrometry