中国河北地区汉族健康人与RA患者padi4_94单核苷酸多态性的研究

Study on single nucleotide polymorphisms of padi4_94 gene in Chinese Han people of Hebei Province

目的研究中国河北地区汉族人群PADI4基因padi4_94位点多态性及其分布特征。方法选取河北地区汉族健康个体106例和类风湿关节炎(RA)患者115例,采用直接测序法对padi4_94位点单核苷酸多态性进行分析,探讨此位点SNP与抗CCP抗体产生的关系。结果本地区汉族人群padi4_94位点存在AA、AG和GG等3种基因型,健康组与RA组的基因频率及等位基因频率无明显区别,且这种SNP并不对RA患者抗CCP抗体的存在产生影响。结论中国河北地区汉族人群padi4_94位点存在多态性,但可能不影响RA的易感性以及抗CCP抗体的产生。

Objective To study the gene polymorphism at position padi4_94 in Chinese Han people of Hebei Province and the characters of distribution. Methods 106 healthy individuals and 115 rheumatoid arthritis patients in Hebei Province were randomly selected. Padi4 _94 gene was screened by sequencing of the promotor. The correlations between the SNP site and anti-cyclic citrullinated peptide anti- body （anti-CCP） was discussed. Results There were three genotypes： AA, AG and GG in Chinese Han people of Hebei Province. No significant differences of gene frequency and allele gene frequency were found between healthy individuals and RA patients, and no correlation between SNP and the production of anti-CCP. Conclusions Hebei Province The gene polymorphisms at position padi4_94 existed in Chinese Han people of Hebei Province, but possibly would not affect RA susceptibility and the production of anti-CCP.