摘要
采用SRAP技术分析了提莫菲维小麦二倍体与野燕麦远缘杂交后代的真实性及其特点。结果表明,22对SRAP引物中,20对引物在双亲间扩增出多态性条带,其多态性比率为71.76%。me4-em1、me4-em3和me3-em33对引物在提莫菲维与二倍体野燕麦杂交的F3株系中扩增出双亲的特异带,表明该F3株系具有双亲的遗传物质,该F3株系是提莫菲维小麦和二倍体野燕麦成功属间杂交的真实杂种后代。在该F3株系的扩增结果中部分双亲带型消失,并且提莫菲维小麦消失的带数远少于野燕麦消失的带数;同时有非父母标记新带型出现。杂种后代DNA序列的这种变化可能有利于新形成异源多倍体小麦的快速进化、遗传协调和遗传稳定性。
The hybrid progenies from wide crosses T. timopheevi × diploid wild oat were analyzed using SRAP markers to prove their truth and understand their characteristics in the present study. Among 22 primer pairs, 20 gave the polymorphic product between both parents, and the average polymorphic ratio was 71.76%. Three primer pairs （me4-em1, me4-em3 and me3-em3） produced specific markers for either parent in the F3 line from wide crosses T. timopheevi × diploid wild oat, indicating that the F3 line contained both parental homologues of some chromosomes, and was true intergeneric hybrid origin. The F3 line lost some parental SRAP markers, and the markers lost from T. timopheevi were far less than those from the wild oat. Meanwhile, some novel SRAP markers were generated. These DNA sequence changes might contribute to the rapid evolution of the newly formed allopolyploid and allow the divergent genomes to act in harmony.
出处
《湖北农业科学》
北大核心
2009年第2期257-259,共3页
Hubei Agricultural Sciences
基金
贵州省农业攻关项目[黔科合NY字(2006)3002B
黔科合NY字[2008]3017号]
贵州省优秀科技教育人才省长基金项目(黔省专合字(2007)19)
教育部春晖计划项目(15001D-3)
