盾叶薯蓣毛状根的诱导与薯蓣皂甙元的生产

Hairy root induction of Dioscorea zingiberensis and diosgenin production

报道盾叶薯蓣形成毛状根诱导的方法及不同毛状根系间生长速率与薯蓣皂甙元含量的差异.结果表明,盾叶薯蓣的幼叶和茎段难被R1601、A4和LBA9402等发根农杆菌菌株诱导出毛状根,但愈伤组织则比较容易;34%的被R1601菌株感染、并在添加200μmol/L乙酰丁香酮的共培养基上培养3 d的愈伤可以在30 d内形成毛状根;不同的毛状根系在生长速率与薯蓣皂甙元含量方面存在显著差异.

Dioscorea zingiberensis is the most important medicinal plant used for production of diosgenin in China. This paper reports that the effects of explant types, strains of Agrobacterium rhizogenes and acetosyringone concentrations on the induction of hairy roots of D. zingiberensis, and the growth rates and diosgenin contents of different hairy root clones. The results showed that hairy roots could not be induced by A. rhizogenes strain R1601, A4 or LBA9402 from both the young leaves and stems of D. zingiberensis, but readily from callus pieces; 34% of the callus explants which were infected with A. rhizogenes and coeultured on the medium supplemented with 200 μmol/L acetosyringone for 3 days produced hairy roots within 30 days; there were significant differences in the growth rates and diosgenin contents among different hairy root clones.