摘要
为了给海岛棉育种实践提供可靠的信息，采用SSR标记技术对14个已知来源的海岛棉品种的遗传背景进行了遗传多样性分析。从筛选出的41对有多态性的SSR引物中共扩增出280个等位位点，其中214个位点有多态性，占76．4％，每对引物可扩增出1～15个位点，平均6．83个等位位点。聚类分析表明，在Ski相似系数0．64水平上14个海岛棉品种可聚成4类。实验证明SSR标记技术可为海岛棉育种中亲本选配。提供可靠的分子水平上的依据。
The genetic relationships among 14 Sea- Island Cotton varieties were analyzed by SSRs to provide credible genetic information in breeding Sea - Island Cotton 280 distinctive loci in total were amplified from 41 pairs of SSR primers , and 214 were polymorphic , which accounted for 76.4 %. 1 to 15 polymorphic loci could be amplified from each primer , with an average of 6.83. The 14 Sea- Island Cotton varieties could be classified into 4 groups at the level of SM = 0.64 by cluster analysis . SSR markers proved to be the work of Sea - Island cotton breeding practice of matching parents , to provide a reliable basis for the molecular level.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2009年第2期237-241,共5页
Xinjiang Agricultural Sciences
基金
新疆生产建设兵团科技局博士资金项目(04BSZJ03)
关键词
海岛棉
SSR
聚类分析
遗传多样性
sea - island cotton
SSR
cluster analysis
genetic diversity
