药用植物刺山柑茎段组织培养研究

Study on Tissue Culture of Stem Segment in Medicinal Plant Capparis spinosa L.

以药用植物刺山柑初秋茎段为外植体,研究了不同灭菌时间对刺山柑无菌外植体建立的影响及不同激素、不同浓度组合对刺山柑丛生芽的诱导、增殖、壮苗和生根的影响。结果表明:外植体灭菌采用0.1%升汞消毒8 min效果最佳,其污染率和死亡率分别为5.6%和6.3%;诱导丛生芽的最适培养基为MS+6-BA 0.6mg/L,其侧芽萌发率可达90%;增殖的最佳培养基为MS+6-BA 0.6 mg/L+IAA 0.2 mg/L,其增殖倍数可达到4.5倍;壮苗培养基以MS+NAA 0.2 mg/L+GA30.2 mg/L效果较好,长势比较旺盛,平均株高、平均芽数均较高;诱导生根的最佳培养基为MS+IBA 0.5 mg/L+0.1%活性炭,生根率达65%,基部几乎无愈伤,且根数较多。

This paper analyzed the influence of different sterilization time on institution of asepsis explant of Capparis spinosa L. and the effects of combinations of various hormone and different concentration on the induction, proliferation, strong seeding, as well as roofing of Capparis spinosa L., using its early autumn stem segment as the explant. The results showed that explant sterilizated with 0.1% HgCl2 for eight minutes were the best, and the pollution rate was about 5.6% and the death rate was about 6.3 %. In addition, the best culture medium for inducing bushy shoots was MS ＋ 6 - BA0.6 mg/L, and the germination rate of lateral bud was up to 90% ; The best culture medium for multiplication was MS ＋ 6 - BA0.6 mg/L ＋ IAA0.2 mg/L, and the multiplication times was about 4.5 more than before. Furthermore, the best culture medium for strong seeding was MS ＋ NAA0.2 mg/L ＋ GA30.2 ing/L, the growth situation of bud was best, the mean height was high and the number of strain greater. For rooting, MS ＋ IBA0.5 mg/L ＋ 0.1% active carbon had a better effect, and the rate of roofing was up to 65%. What＇s more, there were hardly callus and more number of root on the base of bud.