海洋放线菌发酵物对肝癌细胞株生长和凋亡的影响

Effects of marine actinomycete fermented compound on the growth and apoptosis of HepG-2 cells in vitro

目的研究一株南海海洋放线菌的发酵产物对肝癌细胞生长和凋亡的影响。方法采用MTT法评定海洋放线菌的发酵产物对肝癌细胞HepG-2增殖抑制作用及对正常细胞Vero细胞的细胞毒性,经Hoechst33258(8g/L)染色进行形态学观察,应用流式细胞术检测细胞周期、凋亡和凋亡基因Fas的表达情况。结果发酵物对HepG-2细胞的生长抑制效果明显,荧光显微镜下观察发现培养细胞中出现核固缩、凋亡小体;发酵物在诱导HepG-2细胞凋亡同时,下调Bcl-2表达水平,且均呈剂量效应关系。结论发酵物通过阻滞肿瘤细胞的生长及通过抑制Bcl-2诱导细胞凋亡等机制,对肝癌细胞的增殖有一定的抑制作用。

Objective To investigate the influence of Marine Actinomycete Fermented Compound on the growth and apoptosis of HepG -2 cells in vitro and its possible mechanism. Methods The inhibition of Fermented Compound on HepG -2 cells and normal cells（Vero） proliferation was detected by MTT method and disposed cells were stained with Hoechst 33258 （ 8 g/L）. Cell apoptosis and expression of gene Bcl - 2 gene were further analyzed with flow cytometry.Results Fermented Compound significantly inhibited the growth of HepG -2 cells. The karyopycnosis and apoptotic body were observed under fluorescence microscope after ceils were freated with different concentrations of the Fermented Compound. Apoptotic peak and Bcl -2 expression were down regulated as measured by flow cytometry. Conclusion The inhibitory effect of Fermented Compound on HepG - 2 is related to induction of apoptosis by down - regulating Bcl - 2 expression.