摘要
目的观察人参皂甙Rg1对大鼠急性心肌梗死后血管再生和心肌VEGF蛋白表达的影响,并对其作用机制作初步探讨。方法结扎SD大鼠左冠状动脉前降支,将大鼠随机分为Rg1治疗组、单纯手术组及假手术组。手术2、4周后分别测定有关心功能指标,免疫组化方法检测心肌VEGF的表达量,Ⅷ因子和SAM染色观察微血管密度和功能血管再生情况。结果人参皂甙Rg1治疗组心功能好转,心肌微血管密度和功能血管数量明显高于单纯手术组,差异有统计学意义(P<0.01),缺血区和梗死区的范围也明显缩小,差异有统计学意义(P<0.05),VEGF的表达量也高于单纯手术组,差异有统计学意义(P<0.01)。结论人参皂甙Rg1有促进缺血心肌血管生成、保护缺血心肌、缩小梗死面积、改善心功能的作用。
Objective To observe the effects of ginsenoside Rgl on blood vessel regeneration and VEGF expression in rats with acute myocardial infarction. Methods After ligation of the left anterior descending coronary artery,60 surviving male SD rats were randomly assigned into three groups：ginsenoside Rgl treated group,operated group and sham operated group. Two weeks and four weeks later after the operation, heart function were measured respectively. The measurement of capillary density was evaluated using immunostaining for von Willebrand and SAM factor. Immunohistochemisty were used to detect the expression of VEGF. Resuits Capillary density in ginsenoside Rgl treated group was greater than those of operated group and sham -operated groups（P〈0.01）. Infarct area was also obviously less than in operated group（P〈0.05）. Expression of VEGF was found in all groups, but in ginsenoside Rgl treated group the expression of VEGF enhanced obviously（P〈0.01）. Conehtsion Ginsenoside Rgl increases the capillary density after acute myocardial infarction in rats,enhanced myocardial angiogenesis and improved left ventricular function.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第7期805-807,共3页
Chongqing medicine
