摘要
对地表水中的微囊藻毒素-LR进行高效液相色谱法(HPLC)测定,采用V乙醇:V水(含0.1%三氟乙酸)=45:55为流动相,微囊藻毒素-LR在ODS色谱柱上获得理想的分离效果。采用含0.1%三氟乙酸的乙醇溶液在样品前处理提取中获得较高的提取效率,通过优化前处理及检测条件,有效地去除了干扰,获得了简便、安全、有效的检测效果。
The microcystin-LR in the surface water was determined by high-performance liquid chromatography （HPLC） with ethanol ： water （containing 0.1% trifluoroacetic acid） = 45 ： 55 （V / V） as the mobile phase. The result of the separation of MC-LR in the ODS column was satisfied. The use of ethanol solution containing 0.1% trifluoroacetic acid to extract the sample can get a higher recovery of extraction in the pre-treatment process. By optimizing the pre-treatment and conditions of analysis and reducing the interference, a simple, safe and effective results can be obtained.
出处
《广州化学》
CAS
2009年第1期50-53,共4页
Guangzhou Chemistry
关键词
微囊藻毒素
高效液相色谱
流动相
乙醇
microcystins, high-performance liquid chromatography, mobile phase, ethanol
