光叶拟单性木兰挥发油的化学成分及其生物活性的初步研究

Chemical Composition and Biological Activity of Volatile Oil from Parakmeria Nitida

采用水蒸气蒸馏法(SD)提取光叶拟单性木兰叶片中的挥发油,用气相色谱-质谱(GC-MS)联用技术分析挥发油的成分,鉴定出34个化合物。其主要成分为1R-α-蒎烯(7.432%)、香桧烯(13.516%)、β-松节烯(7.615%)、间-甲基异丙基苯(7.21%)、桉油醇(7.139%)、松油-4-醇(5.004%)、β-石竹烯(5.577%)、β-荜澄茄烯(4.435%)。体外抑菌和抗肿瘤实验表明,光叶拟单性木兰挥发物质对大肠杆菌、金黄色葡萄球菌、红酵母有一定的抑制作用;对人非小细胞肺癌(NCI-H460)、胃腺癌(SGC-7901)有较强的抑制作用,当挥发油质量浓度为50μg.mL-1时,抑制率分别达到83.24%、72.26%。

The volatile oil was extracted from the leaves of Parakmeria nitida by steam distillation and the chemical constituents were analyzed by GC-MS method.34 compounds were identified. The main compounds included 1R-α-pinene（7.432% ）, Sabinene （13. 516% ）, β-Pinene（7. 615% ）, m-Cymene（7.21% ）, eudesmol（7. 139% ）, oleum pini pumilionis-4-alcohol（5. 004% ）, β- caryophyllene（5. 577 % ）, and β-Cuvebene（4. 435 % ）. The antibacterial and anti-knub experiments showed that the oil had some certain control actions to staphylococcus aureus, escherichia coli, rhodotorula glutinis. The control action to NCI-H460 and SGC7901 were relatively strong. The control rate were 83.24 % and 72.26% at the volatile oil concentration of 50μg·mL^-1.