肉鸡肠道CDX2 mRNA表达的肠段差异性与发育性变化

Regional and Ontogenetic Expression of CDX2 mRNA in Intestine of Chicken

选用遗传背景相同的1日龄父母代雄性Arbor Acre(AA)肉雏鸡120羽,随机分为4个重复,采用相对定量RT-PCR方法,以AA肉鸡30 d肠道样品为模板,研究肉鸡肠道尾形相关同源盒基因(cadua-related type homeoboxgene)家族中CDX2 mRNA表达的组织特异性;以AA肉鸡十二指肠和空肠样品为模板,研究肉鸡肠道CDX2 mRNA表达的发育性变化.结果显示:AA肉鸡十二指肠CDX2 mRNA的表达丰度高于空肠(P=0.09)、回肠(P=0.06)和结直肠(P=0.05);AA肉鸡CDX2 mRNA在十二指肠及空肠中的表达具有相同的发育模式,2～30 d下降,44 d回升,58 d略微下降;在2和44 d时的表达丰度显著高于30 d(P<0.05).以上结果表明:AA肉鸡肠道近端CDX2mRNA的表达丰度高于远端(P>0.05).AA肉鸡十二指肠及空肠CDX2 mRNA的表达具有相同的发育模式,表明CDX2 mRNA表达受到发育阶段的调控,且在十二指肠和空肠间具有稳定性.

One hundred and twenty 1-day-old parental male Arbor Acre （AA） broiler chicks were randomly divided into 4 replicates, respectively. Regional expression of CDX2 mRNA in different segments of intestinal tract of AA chicken on 30 d and ontogenetic expression of CDX2 mRNA in duodenum and jejunum of AA broiler chickens were determined by relative quantitative RT-PCR. It was found that： The CDX2 mRNA expression in duodenum was higher than that in jejunum （ P = 0.09 ）, ileum （ P = 0.06） and colorectum （P=0.05 ） , respectively. Ontogenetic patterns of CDX2 mRNA were consistent between duodenum and jejunum. The abundance of CDX2 mRNA decreased from day 2 to day 30, increased from day 30 to day 44 and increased slightly on day 58. The abundance of CDX2 mRNA on day 2 and day 44 were higher than on day 30 （P 〈 0.05 ） , respectively. These results indicate that： Regional expression of CDX2 mRNA in intestine of AA chickens was declined along the downward intestine from proximal intestine to distal intestine （P 〉 0.05）. Ontogeny of CDX2 mRNA in AA chickens had the same pattern in duodenum and jejunum, indicating that CDX2 mRNA expression could be regulated by developmental stage.