摘要
目的探讨抗衰老中药方剂补肾填精方对D-半乳糖衰老模型大鼠脑细胞周期相关p53、p21和p16蛋白表达的影响。方法采用D-半乳糖法诱导衰老大鼠模型,随机分为正常组、模型组、天保宁组、补肾填精方(低剂量组、中剂量组、高剂量组)。免疫组化法检测各组大鼠脑海马CA1区细胞周期相关p53、p21和p16蛋白表达。结果补肾填精方能抑制海马CA1区细胞周期相关p53、p21和p16阳性蛋白的表达(P<0.05)。结论补肾填精方可降低衰老大鼠海马CA1区p53、p21和p16蛋白表达,提示补肾填精方延缓脑细胞衰老可能与细胞周期相关蛋白表达相关。
Objective To observe the effect of anti age herbal prescription nourishing kidney-essence formula on p53, p21 and p16 expression in D-gatactose induced senile rats. Methods Senile rats were induced by D-galactos, and divid ed into 6 groups ： a normal group, a model group, a Tianbaoning group, and 3 nourishing-kidney-essence herb groups （low-dose. middle-dose, and high dose）. Expressions of p53, p21 and p16 in CA1 hippocampus region were quantified by immunohistochemistry. Results Nourishing kidney-essence herbs could inhibit cell cycle related p53, p21 and p16 protein expressions （P〈0.05）. Conclusion Nourishing kidney-essence herbs could inhibit cell cycle related gene p53. p21 and p16 expression, which may be related to the anti age effect of nourishing kidney essence herbs.
出处
《中南药学》
CAS
2009年第3期161-164,共4页
Central South Pharmacy
基金
上海市教委青年基金项目(基金编号:04CC19)
