高效液相色谱法测定肿节风药材中异秦皮啶与山柰酚-3-O-葡萄糖醛酸苷的含量

Determination of isofraxidin and kaempferol-3-O-glucuronide in herba sarcandrae by HPLC

目的建立肿节风药材中山柰酚-3O-葡萄糖醛酸苷与异秦皮啶的含量测定方法。方法色谱柱为Kromasil ODS-1（4．6mm×250mm，5μm），流动相为乙腈-0．1％磷酸水溶液，梯度洗脱，流速为1．0mL·min^-1，检测波长为344nm，柱温为25℃。结果山柰酚-3-O-葡萄糖醛酸苷在0．2006～4．012μg线性关系良好，相关系数r=0．9999．平均回收率为100．1％，RSD=1．8％；异秦皮啶在0．4352～8．704μg线性关系良好，相关系数r=0．9999．平均回收率为99．8％，RSD=1．5％。结论所用方法简便、准确，重复性好，在同一高效液相色谱条件下可同时测定山柰酚-3-O-葡萄糖醛酸苷和异秦皮啶的含量，提供了更好的控制肿节风药材质量的方法。

Objective To establish a method for the determination of isofraxidin and kaempferol- 3- O-glucuronide in herba sarcandrae. Methods The separation was performed on a Kromasil ODS- 1 （4.6 mm〉（250 mm, 5 μm）. The mobile phase consisted of ACN -0.1% H3PO4, and gradient elution was used. The flow rate was 1.0 mL·min^-1. The detection wavelength was 344 nm and the column temperature was 25 C. Results The calibration curves of kaempferol- 3O-glucuronide were in good linearity at 0. 200 6-4. 012 μg （r=0. 999 9）. The average recovery was 100.1% with RSD of 1.8%. The calibration curves of isofraxidin were in good linearity at 0. 435 2-8. 704μg （r=0. 999 9） The average recovery was 99.8% with RSD of 1.5%. Conclusion This method is simple, accurate and repeatable. It can be used to simultaneously determine kaempferol 3-O-glucuronide and isofraxidin with the same chromatogram condition. It provides a better quality control method for herba sarcandrae.