高效液相色谱法测定六味补血颗粒中芍药苷和阿魏酸的含量

Content determination of paeoniflorin and ferulic acid in Liuwei Buxue granules by HPLC

目的建立HPLC法测定六味补血颗粒中芍药苷和阿魏酸的含量。方法采用UltimateXB-C18柱（250min×4．6mm，5μm）；流动相为乙腈-0．1％磷酸溶液（15：85）；检测波长232nm（芍药苷）、323nm（阿魏酸）；流速：1．0mL·min^-1；柱温：25℃。结果芍药苷和阿魏酸的线性范围分别为0．108-0．864μg（r=0.9996，n=6）和0．0440～0．352μg（r=0．9998，n=6）；芍药苷和阿魏酸的平均回收率分别为97．77％和96．78％，RSD（n=9）分别为1．33％和1．08％。结论该方法可用于六味补血颗粒中芍药苷和阿魏酸的合量测定．

Objective To establish a method for the content determination of paeoniflorin and ferulic acid in Liuwei Buxue granules by HPLC. Methods Ultimate XB-C18 column （250 mm × 4.6 mm, 5μm） was used. The mobile phase was acetonitrile-0. 1% phosphoric acid solution （15 ： 85）, the detection wavelenghth was 230 nm for paeoni- florin and 323 nm for ferulic acid, the flow rate was 1.0 mL·min^-1, and column temperature was 25℃. Results The linear range of paeoniflorin and ferulic acid was 0. 108-0. 864μg （r=0. 999 6, n=6 ） and 0. 044 0-0. 352 μg （r=0. 999 8, n=6 ）. The average recovery of paeoniflorin and ferulic acid was 97. 77% and 96.78%, RSD （n=9） was 1.33% and 1.08%. Conclusion The method may be used for the content determination of paeoniflorin and ferulic acid in Liuwei Buxue granules.