乙肝五项时间分辨免疫荧光分析与PCR-HBV定量的关系

目的研究乙肝血清标志物时间分辨免疫定量检测结果与HBV-DNA定量之间的关系。方法将200例血清标本同时采用时间分辨免疫荧光定量和实时荧光定量PCR技术,对乙肝五项含量和HBV-DNA含量分别进行检测,按照不同的乙肝病毒标志模式情况分组后,进行乙肝病毒标志物与HBV-DNA的分析研究。结果乙肝病毒标志物HBsAg、HBeAg、HBcAb阳性组HBV-DNA检出率为98.7%,HBsAg、HBeAb、HBcAb阳性组检出率59.3%,HBcAb单项阳性组检出率50%,HBsAb阳性组检出率0,全阴性组检出率0。结论时间分辨免疫荧光分析与HBV-DNA关系密切,两者联用能更好的满足临床需求。

Objective To investigate the relationships between HBV infected patients＇immunological marker detected by time-resolved fluoroimmunoassay（TRFIA）quantitatively and HBV-DNA concentrations.Methods Two hundred samples were detected by TRFIA and real-time quantitative PCR simultaneously.According to different HBV marker pattern,results were divided into several categories and analyzed comparatively with HBV-DNA level.Results In patients with HBsA（g＋）/HBeAg（＋）/HBcAb（＋）,HBsAg（＋）/HBeAb（＋）/HBcAb（＋）,HBcAb（＋）,HBsAb（＋）and HBV-M-negative,the HBV-DNA positive rate were 98.7%,59.3%,50%,0,0,respectively.Conclusion TRFIA and HBV-DNA had a close relationship.TRFIA in combination with HBV-DNA can meet the need of Clinic better.