不饱和脂酸及其硒化物对BEL-7402人肝癌细胞的杀伤作用及DNA合成的影响

Cytotoxic Action and Effect of Unsaturated Fatty Acids and Their Selenides on DNA Synthesis in BEL 7402 Human Hepatocarcinoma Cells

研究了亚麻酸、亚油酸、油酸及其硒化物对人肝癌细胞杀伤作用及对ＤＮＡ合成的影响。结果表明，三种脂酸及其硒化物对ＢＥＬ－７４０２人肝癌细胞均有杀伤作用，其中以硒化亚麻酸杀伤作用最强，５００μｇ／ｍｌ即可杀灭全部肝癌细胞，且具剂量效应关系。其次是亚麻酸、亚油酸和硒化亚油酸。油酸和硒化油酸杀伤作用较弱。１５０μｇ／ｍｌ的亚麻酸、亚油酸及其硒化物和硒化油酸对肝癌细胞的ＤＮＡ合成均有明显抑制作用（Ｐ＜０．０１），其中以硒化亚麻酸抑制作用最强（Ｐ＜０．０５～０．０１），且具有剂量效应关系。亚麻酸、亚油酸对ＤＮＡ合成的抑制作用与相应的硒化物之间无明显差异（Ｐ＞０．０５），而只含一个双键的油酸对ＤＮＡ合成无明显抑制作用，其硒化物则有显著抑制作用，两者的差异非常显著（Ｐ＜０．０１）。

The cytotoxic action and effect of unsaturated fatty acids linolenic acid, linoleic acid, oleic acid and their selenides on DNA synthesis of BEL 7402 human hepatocarcinoma cells were studied. The results showed that all these fatty acids exhibited cytotoxic action on BEL 7402 cells which was dose related. Selenide of linolenic acid was the strongest toxic and all cells died at the concentration of 500μg/ml. The oleic acid and its selenide were the weakest. The inhibition of unsaturated fatty acids and their selenides on DNA synthesis in BEL 7402 cells was also studied by the H TdR incorporation. It was demonstrated that selenide of linolenic acid was the strongest inhititor of DNA synthesis among these fatty acids when the concentration was 150μg/ml as compared with linolenic acid ( P <0.05), oleic acid ( P <0.01), selenide of lenoleic acid and oleic acid ( P <0.01). There was no remarkable difference in inhibition between lenolenic acid and its selenide ( P <0.05). But the difference between oleic acid and its selenide was significant ( P <0.01).