当归多糖及当归内酯对小鼠细胞免疫功能的影响

THE EFFECT OF DANG GUI POLYSACCHARIDES AND DANG GUI LACTONE ON CELLULAR IMMUNITY IN MICE

利用混合淋巴细胞培养反应（ＭｉｘｅｄＬｙｍｐｈｏｃｙｔｅＲｅａｃｔｉｏｎ，ＭＬＲ）研究了传统中药当归（ＡｎｇｅｌｉｃａＳｉｎｅｎｓｉｓＤｉｅｌｓ，ＡＳＤ）来源的两种免疫调节剂当归多糖（ＡＳＤＰ）、当归内酯（ＡＳＤＥ３）的免疫作用机理。结果表明：两者均能明显促进由同种异型抗原刺激的正常小鼠及Ｓ１８０荷瘤小鼠脾细胞增殖（Ｐ＜０．０１），其促进作用与剂量之间呈依赖关系。在一定浓度范围内（１６～５００μｇ／ｍｌ）对未经活化的脾细胞无诱导增殖作用。选择促进效应较大的药物剂量，培养９６ｈ，对活化的Ｔ淋巴细胞亚群的变化标记检测，结果显示：ＡＳＤＰ可明显增加Ｌ３Ｔ＋４细胞比例，ＡＳＤＥ３可明显增加Ｌ３Ｔ＋４和Ｌｙｔ＋２细胞的数量。在浓度为２５０μｇ／ｍｌ时，ＡＳＤＥ３增强细胞毒Ｔ细胞的功能，其杀伤活性增加８０％。

おSDP and ASDE3 are extracted from traditional Chinese MedicineAngelica Sinensis Diels (ASD). Their immunopharmacological effects have been studied. Mixed Lymphocyte Reaction was used as a main model through all the experiment. In a series of concentration, ASDP and ASDE3 markedly promoted proliferation of normal mouse and S180 bearing tumor mouse splenocytes stimulated by alloantigens in vitro (P<0.01). The effects were positively correlated with concentration of the agents. In certain range of concentration (16～500 μg/ml), ASDP and ASDE3 could not induce proliferation of inactivated mouse splenocytes. T lymphocyte subsets were assessed after treatment with ASDP and ASDE3 in the optimal concentration, meanwhile take fresh and alloantigen activated lymphocyte as control. It is observed that the L3T+4 cells were increased obviously by treated with ASDP, L3T+4 cells and Lyt+2 cells were also increased markedly by treated with ASDE3 in vitro. The data also show that ASDE3 markedly enhanced the cytotoxicity of cytotoxic T lymphocytes, which was increased 80% at the concentration of 250 μg/ml in vitro.