亮氨酸甲基酯处理法制备抗人肾癌单克隆抗体

UTILIZATION OF LEUCINE METHYL ESTER FOR THE GENERATION OF HYBRIDOMAS PRODUCING MONOCLONAL ANTIBODIES SPECIFIC TO KIDNEY AND SUBSETS OF RENAL CELL CARCINOMAS

以本所自建的肾癌细胞株（ＧＲＣ１）作为免疫原，用亮氨酸甲基酯（ＬｅｕＯＭｅ）处理免疫后的脾细胞，可以显著提高细胞融合率，并且筛选到４株抗人肾癌单克隆抗体（ＭＡｂ）。ＹＤ５为其中特异性较好的１株，属ＩｇＧ１亚类，在正常肾组织的肾小管、大多数肾癌（１３／１５）组织中表达，且与其它被检正常组织交叉反应少。当以某些肿瘤细胞株作为免疫原且融合率很低时。

おy treating the primed spleen cells with leucine methyl ester (LeuOMe),4 murine monoclonal antibodies (MAbs) was generated following immunization of mice with our own established human renal cancer cell line GRC1.LeuOMe can drastically improved the outgrowth of hybridomas.YD5,an IgG1 murine Mab,was of special insterest.YD5 is highly reactive with normal kidney and human renal cancer,and has little crossreactivity to nonrenal tissue.This procedure will be an option when production of MAb specific to cellsurface antigen is intended and outgrowth of hybridoma is unexpectedly low.