免疫磁性微珠在HCG定量检测中的批间稳定性研究

THE STABILITY STUDY OF MAGNETIC MICROBEAD AMONG THE GROUPS IN HCG QUANTITATIVE DETECTION

用悬浮聚合法制备了六批磁性微珠，其表面有相同量的胺基功能基团；用戊二醛一步法，对αＨＣＧ单抗标记了碱性磷酸酶，用βＨＣＧ单抗制备了磁珠一抗，测得ＨＣＧ浓度为５００ｍＵ／ｍｌ、２５０ｍＵ／ｍｌ、１００ｍＵ／ｍｌ时的ＯＤ值ＣＶ偏差分别为４６％、６８％和７０％。

? Prepared 6 groups magnetic beads with suspended polymerization, there are the same amounts of amino functional groups in the surfaces of IMBS. The αHCG antibodies were conjugated with the alkaline phosphatase by glutaraldehyde onestep method, and the βHCG antibodies bound to the IMBS with soft chemical reaction. The CIA test demonstrated that the coefficient of variation of absorption at 550 nm respectively is 4.6%, 6.8% and 7.0% when the HCG respective concentration is 500 mU/ml, 250 mU/ml and 100 mU/ml.