摘要
目的探索缺氧诱导因子-1α(H IF-1α)和血红素氧化酶-1(HO-1)在糖尿病肾病大鼠肾组织中的表达情况,及其与糖尿病肾脏微血管病变的关系。方法用链脲佐菌素(STZ)制作糖尿病肾病大鼠模型,在制模成功后2、4、8、12、16周取肾脏组织,以年龄匹配正常大鼠作为对照组,采用免疫组织化学SP方法结合图像分析检测糖尿病肾病大鼠石蜡切片中H IF-1α与HO-1的表达量。结果免疫组化显示H IF-1α在两组均有表达,但两组比较有差异(P<0.01)。HO-1在对照组无明显表达,但模型组有明显表达,两组比较有差异(P<0.01)。模型组H IF-1α和HO-1表达量均于8周时达高峰。模型组肾小管上皮细胞胞浆中H IF-1α和HO-1表达量呈正相关。结论糖尿病肾病存在H IF-1α和HO-1的表达上调,糖尿病肾脏存在缺氧状态,其缺氧状态可能与其微血管结构的改变有关,并参与了糖尿病肾病的发生发展。
Objective To explore the expression levels of hypoxia inducible factor -1α （HIF - 1α） and heine oxygenase- 1 （HO- 1 ） and the relation between them and microvascular pathologic changes in kidney tissue of rats with diabetic nephropathy. Methods The kidneys were obtained from streptozotocin （STZ） -induced diabetic rats at 2,4,8 ,12,16 weeks and rats as age -matched control group, respectively. Then the expression levels of HIF - 1α and HO - 1 were determined with immunohistochemistry SP detection methods and image analysis in renal paraffin section of rats with diabetic nephropathy. Results Immunohistochemistry showed that HIF - 1α was expressed in STZ - induced diabetic rats and the control group and the difference was significant between them （P 〈0.01）. The expression of HO- 1 was very low in the control group and high in the model group, and the difference was significant （P 〈0.01）. The expression levels of HIF - 1α and HO - 1 reached thepeak at 8 weeks in in the model group. The expression of HIF -1α and HO -1 was direct correlation in tubular epithelial cells of the model group. Conclusion The expression of HIF -1α and HO- 1 are upregulated in the diabetic nephropathy. The hypoxic condition exists in diabetic nephropathy, and it is connected possibly with the renal microvascular dysfunction and development of the diabetic nephropathy.
出处
《湖北民族学院学报（医学版）》
2009年第1期5-7,共3页
Journal of Hubei Minzu University(Medical Edition)
基金
贵州省优秀科技教育人才省长资金项目(2006.136)
