四虫片对低氧培养人肝癌HepG2细胞株增殖和凋亡的影响

The Effects of Sichong Pill on Proliferation and Apoptosis of Hepatoma Cell Line HepG2

目的观察四虫片含药血清对人肝癌HepG2细胞增殖的抑制作用,以及对肝癌细胞突变型p53表达的影响。方法用不同浓度的四虫片和氟尿嘧啶(5-FU)分别加入肝癌细胞株中一起培养。用单四唑(MTT)法检测其对肝癌细胞增殖的抑制作用,用PV-9000二步法对突变型p53表达进行测定。结果MTT检测显示四虫片高浓度(25%)含药血清72 h对肝癌细胞的抑制率为53%,5-FU(10μg/m l)对照组抑制率为22%,两组有明显差异(P<0.05)。经药物处理后,两组所测变异型p53表达差异明显(P<0.05),HOECHST染色试剂盒所测细胞凋亡率亦有明显差异(P<0.05)。结论四虫片能有效抑制肝癌细胞增殖,且能诱导肝癌细胞凋亡。其诱导肝癌细胞凋亡的机制可能与下调突变型p53蛋白的表达有关。

Objective To investigate the vitro inhibitory effects of Sichong Pill contained blood serum on the proliferation of hepatoma cell line HepG2 , and to determine the expression of the gene 1353 in HepG2. Methods The inhibition （ 10% ,15% ,20% ,25%） and 5 - FU（ 10 μg/ml） ratio of Sichong Pill at different concentrations to HepG2 was determined by using MTT after 24h, 48h and 72h, respectively and the expression of p53 was examined by the use of PV - 9000 two -step. Results The inhibition ratio of 25% Sichong Pill contained blood serum and 5 - FU of 10μg/ml was 53% and 22% in HepG2, respectively. And the differences in inhibition ratio, p53 expression and the rate of apoptosis were significant between two groups （P 〈 0.05）. Conclusions Sichong Pill can inhibit the growth of hepatoma cells and induce apoptosis perhaps by the way of decreasing the expression of the p53 protein.